diff --git a/django/core/checks/__init__.py b/django/core/checks/__init__.py
index 0aa23dbaf4..f2194a3fc9 100644
--- a/django/core/checks/__init__.py
+++ b/django/core/checks/__init__.py
@@ -9,7 +9,6 @@ from .registry import Tags, register, run_checks, tag_exists
 
 # Import these to force registration of checks
 import django.core.checks.caches  # NOQA isort:skip
-import django.core.checks.compatibility.django_1_10  # NOQA isort:skip
 import django.core.checks.database  # NOQA isort:skip
 import django.core.checks.model_checks  # NOQA isort:skip
 import django.core.checks.security.base  # NOQA isort:skip
diff --git a/django/core/checks/compatibility/django_1_10.py b/django/core/checks/compatibility/django_1_10.py
deleted file mode 100644
index 120ef0b5b1..0000000000
--- a/django/core/checks/compatibility/django_1_10.py
+++ /dev/null
@@ -1,17 +0,0 @@
-from __future__ import unicode_literals
-
-from django.conf import settings
-
-from .. import Tags, Warning, register
-
-
-@register(Tags.compatibility)
-def check_duplicate_middleware_settings(app_configs, **kwargs):
-    if settings.MIDDLEWARE is not None and hasattr(settings, 'MIDDLEWARE_CLASSES'):
-        return [Warning(
-            "The MIDDLEWARE_CLASSES setting is deprecated in Django 1.10 "
-            "and the MIDDLEWARE setting takes precedence. Since you've set "
-            "MIDDLEWARE, the value of MIDDLEWARE_CLASSES is ignored.",
-            id='1_10.W001',
-        )]
-    return []
diff --git a/docs/ref/checks.txt b/docs/ref/checks.txt
index 51083c5b2b..13ef44053e 100644
--- a/docs/ref/checks.txt
+++ b/docs/ref/checks.txt
@@ -116,7 +116,8 @@ that might occur as a result of a version upgrade.
   in Django 2.0*.
 * **1_10.W001**: The ``MIDDLEWARE_CLASSES`` setting is deprecated in Django
   1.10  and the :setting:`MIDDLEWARE` setting takes precedence. Since you've
-  set ``MIDDLEWARE``, the value of ``MIDDLEWARE_CLASSES`` is ignored.
+  set ``MIDDLEWARE``, the value of ``MIDDLEWARE_CLASSES`` is ignored. *This
+  check was removed in Django 2.0*.
 
 Caches
 ------
diff --git a/tests/check_framework/tests_1_10_compatibility.py b/tests/check_framework/tests_1_10_compatibility.py
deleted file mode 100644
index 388ac1b024..0000000000
--- a/tests/check_framework/tests_1_10_compatibility.py
+++ /dev/null
@@ -1,17 +0,0 @@
-from django.core.checks.compatibility.django_1_10 import \
-    check_duplicate_middleware_settings
-from django.test import SimpleTestCase
-from django.test.utils import override_settings
-
-
-class CheckDuplicateMiddlwareSettingsTest(SimpleTestCase):
-
-    @override_settings(MIDDLEWARE=[], MIDDLEWARE_CLASSES=['django.middleware.common.CommonMiddleware'])
-    def test_duplicate_setting(self):
-        result = check_duplicate_middleware_settings(None)
-        self.assertEqual(result[0].id, '1_10.W001')
-
-    @override_settings(MIDDLEWARE=None)
-    def test_middleware_not_defined(self):
-        result = check_duplicate_middleware_settings(None)
-        self.assertEqual(len(result), 0)