diff --git a/django/contrib/auth/backends.py b/django/contrib/auth/backends.py
index 4adcf35051..dd3c2e527b 100644
--- a/django/contrib/auth/backends.py
+++ b/django/contrib/auth/backends.py
@@ -1,10 +1,6 @@
-import warnings
-
 from django.contrib.auth import get_user_model
 from django.contrib.auth.models import Permission
 from django.db.models import Exists, OuterRef, Q
-from django.utils.deprecation import RemovedInDjango50Warning
-from django.utils.inspect import func_supports_parameter
 
 UserModel = get_user_model()
 
@@ -211,19 +207,7 @@ class RemoteUserBackend(ModelBackend):
                 user = UserModel._default_manager.get_by_natural_key(username)
             except UserModel.DoesNotExist:
                 pass
-
-        # RemovedInDjango50Warning: When the deprecation ends, replace with:
-        #   user = self.configure_user(request, user, created=created)
-        if func_supports_parameter(self.configure_user, "created"):
-            user = self.configure_user(request, user, created=created)
-        else:
-            warnings.warn(
-                f"`created=True` must be added to the signature of "
-                f"{self.__class__.__qualname__}.configure_user().",
-                category=RemovedInDjango50Warning,
-            )
-            if created:
-                user = self.configure_user(request, user)
+        user = self.configure_user(request, user, created=created)
         return user if self.user_can_authenticate(user) else None
 
     def clean_username(self, username):
diff --git a/docs/releases/5.0.txt b/docs/releases/5.0.txt
index a5c5916511..ed82f62640 100644
--- a/docs/releases/5.0.txt
+++ b/docs/releases/5.0.txt
@@ -324,3 +324,6 @@ to remove usage of these features.
   objects without providing the ``chunk_size`` argument is no longer allowed.
 
 * Passing unsaved model instances to related filters is no longer allowed.
+
+* ``created=True`` is required in the signature of
+  ``RemoteUserBackend.configure_user()`` subclasses.
diff --git a/tests/auth_tests/test_remote_user.py b/tests/auth_tests/test_remote_user.py
index 9e6e0dce20..d923f5d60b 100644
--- a/tests/auth_tests/test_remote_user.py
+++ b/tests/auth_tests/test_remote_user.py
@@ -6,14 +6,7 @@ from django.contrib.auth.backends import RemoteUserBackend
 from django.contrib.auth.middleware import RemoteUserMiddleware
 from django.contrib.auth.models import User
 from django.middleware.csrf import _get_new_csrf_string, _mask_cipher_secret
-from django.test import (
-    Client,
-    TestCase,
-    ignore_warnings,
-    modify_settings,
-    override_settings,
-)
-from django.utils.deprecation import RemovedInDjango50Warning
+from django.test import Client, TestCase, modify_settings, override_settings
 
 
 @override_settings(ROOT_URLCONF="auth_tests.urls")
@@ -279,34 +272,6 @@ class RemoteUserCustomTest(RemoteUserTest):
         self.assertEqual(newuser.email, "user@example.com")
 
 
-# RemovedInDjango50Warning.
-class CustomRemoteUserNoCreatedArgumentBackend(CustomRemoteUserBackend):
-    def configure_user(self, request, user):
-        return super().configure_user(request, user)
-
-
-@ignore_warnings(category=RemovedInDjango50Warning)
-class RemoteUserCustomNoCreatedArgumentTest(RemoteUserTest):
-    backend = "auth_tests.test_remote_user.CustomRemoteUserNoCreatedArgumentBackend"
-
-
-@override_settings(ROOT_URLCONF="auth_tests.urls")
-@modify_settings(
-    AUTHENTICATION_BACKENDS={
-        "append": "auth_tests.test_remote_user.CustomRemoteUserNoCreatedArgumentBackend"
-    },
-    MIDDLEWARE={"append": "django.contrib.auth.middleware.RemoteUserMiddleware"},
-)
-class RemoteUserCustomNoCreatedArgumentDeprecationTest(TestCase):
-    def test_known_user_sync(self):
-        msg = (
-            "`created=True` must be added to the signature of "
-            "CustomRemoteUserNoCreatedArgumentBackend.configure_user()."
-        )
-        with self.assertWarnsMessage(RemovedInDjango50Warning, msg):
-            self.client.get("/remote_user/", **{RemoteUserTest.header: "newuser"})
-
-
 class CustomHeaderMiddleware(RemoteUserMiddleware):
     """
     Middleware that overrides custom HTTP auth user header.