diff --git a/django/db/models/functions/comparison.py b/django/db/models/functions/comparison.py
index 6db81d6f46..d06f0a25a4 100644
--- a/django/db/models/functions/comparison.py
+++ b/django/db/models/functions/comparison.py
@@ -175,7 +175,10 @@ class JSONObject(Func):
         )
 
     def as_postgresql(self, compiler, connection, **extra_context):
-        if not connection.features.is_postgresql_16:
+        if (
+            not connection.features.is_postgresql_16
+            or connection.features.uses_server_side_binding
+        ):
             copy = self.copy()
             copy.set_source_expressions(
                 [
diff --git a/docs/releases/5.1.2.txt b/docs/releases/5.1.2.txt
index 1cf3f9df09..6d2714df85 100644
--- a/docs/releases/5.1.2.txt
+++ b/docs/releases/5.1.2.txt
@@ -12,3 +12,6 @@ Bugfixes
 * Fixed a regression in Django 5.1 that caused a crash when using the
   PostgreSQL lookup :lookup:`trigram_similar` on output fields from ``Concat``
   (:ticket:`35732`).
+
+* Fixed a regression in Django 5.1 that caused a crash of ``JSONObject()``
+  when using server-side binding with PostgreSQL 16+ (:ticket:`35734`).