diff --git a/django/contrib/sitemaps/views.py b/django/contrib/sitemaps/views.py
index 679f7ed611..d696639932 100644
--- a/django/contrib/sitemaps/views.py
+++ b/django/contrib/sitemaps/views.py
@@ -1,5 +1,4 @@
 import datetime
-import warnings
 from dataclasses import dataclass
 from functools import wraps
 
@@ -9,7 +8,6 @@ from django.http import Http404
 from django.template.response import TemplateResponse
 from django.urls import reverse
 from django.utils import timezone
-from django.utils.deprecation import RemovedInDjango50Warning
 from django.utils.http import http_date
 
 
@@ -18,15 +16,6 @@ class SitemapIndexItem:
     location: str
     last_mod: bool = None
 
-    # RemovedInDjango50Warning
-    def __str__(self):
-        msg = (
-            "Calling `__str__` on SitemapIndexItem is deprecated, use the `location` "
-            "attribute instead."
-        )
-        warnings.warn(msg, RemovedInDjango50Warning, stacklevel=2)
-        return self.location
-
 
 def x_robots_tag(func):
     @wraps(func)
diff --git a/docs/releases/5.0.txt b/docs/releases/5.0.txt
index 9fc612f418..f5b4f532a9 100644
--- a/docs/releases/5.0.txt
+++ b/docs/releases/5.0.txt
@@ -305,4 +305,4 @@ to remove usage of these features.
 See :ref:`deprecated-features-4.1` for details on these changes, including how
 to remove usage of these features.
 
-* ...
+* The ``SitemapIndexItem.__str__()`` method is removed.
diff --git a/tests/sitemaps_tests/templates/custom_sitemap_index.xml b/tests/sitemaps_tests/templates/custom_sitemap_index.xml
deleted file mode 100644
index 406c6b7606..0000000000
--- a/tests/sitemaps_tests/templates/custom_sitemap_index.xml
+++ /dev/null
@@ -1,5 +0,0 @@
-<?xml version="1.0" encoding="UTF-8"?>
-<!-- This is a customised template -->
-<sitemapindex xmlns="http://www.sitemaps.org/schemas/sitemap/0.9">
-{% for location in sitemaps %}<sitemap><loc>{{ location }}</loc></sitemap>{% endfor %}
-</sitemapindex>
diff --git a/tests/sitemaps_tests/test_http.py b/tests/sitemaps_tests/test_http.py
index 1a0c1c3d50..74d183a7b0 100644
--- a/tests/sitemaps_tests/test_http.py
+++ b/tests/sitemaps_tests/test_http.py
@@ -4,9 +4,8 @@ from datetime import date
 from django.contrib.sitemaps import Sitemap
 from django.contrib.sites.models import Site
 from django.core.exceptions import ImproperlyConfigured
-from django.test import ignore_warnings, modify_settings, override_settings
+from django.test import modify_settings, override_settings
 from django.utils import translation
-from django.utils.deprecation import RemovedInDjango50Warning
 from django.utils.formats import localize
 
 from .base import SitemapTestsBase
@@ -579,44 +578,3 @@ class HTTPSitemapTests(SitemapTestsBase):
         </sitemapindex>
         """
         self.assertXMLEqual(index_response.content.decode(), expected_content_index)
-
-
-# RemovedInDjango50Warning
-class DeprecatedTests(SitemapTestsBase):
-    @override_settings(
-        TEMPLATES=[
-            {
-                "BACKEND": "django.template.backends.django.DjangoTemplates",
-                "DIRS": [os.path.join(os.path.dirname(__file__), "templates")],
-            }
-        ]
-    )
-    def test_simple_sitemap_custom_index_warning(self):
-        msg = (
-            "Calling `__str__` on SitemapIndexItem is deprecated, use the `location` "
-            "attribute instead."
-        )
-        with self.assertRaisesMessage(RemovedInDjango50Warning, msg):
-            self.client.get("/simple/custom-index.xml")
-
-    @ignore_warnings(category=RemovedInDjango50Warning)
-    @override_settings(
-        TEMPLATES=[
-            {
-                "BACKEND": "django.template.backends.django.DjangoTemplates",
-                "DIRS": [os.path.join(os.path.dirname(__file__), "templates")],
-            }
-        ]
-    )
-    def test_simple_sitemap_custom_index(self):
-        "A simple sitemap index can be rendered with a custom template"
-        response = self.client.get("/simple/custom-index.xml")
-        expected_content = """<?xml version="1.0" encoding="UTF-8"?>
-    <!-- This is a customised template -->
-    <sitemapindex xmlns="http://www.sitemaps.org/schemas/sitemap/0.9">
-    <sitemap><loc>%s/simple/sitemap-simple.xml</loc></sitemap>
-    </sitemapindex>
-    """ % (
-            self.base_url
-        )
-        self.assertXMLEqual(response.content.decode(), expected_content)
diff --git a/tests/sitemaps_tests/urls/http.py b/tests/sitemaps_tests/urls/http.py
index 2b512cfd69..db549b4a38 100644
--- a/tests/sitemaps_tests/urls/http.py
+++ b/tests/sitemaps_tests/urls/http.py
@@ -279,11 +279,6 @@ urlpatterns = [
         views.index,
         {"sitemaps": simple_sitemaps_not_callable},
     ),
-    path(
-        "simple/custom-index.xml",
-        views.index,
-        {"sitemaps": simple_sitemaps, "template_name": "custom_sitemap_index.xml"},
-    ),
     path(
         "simple/custom-lastmod-index.xml",
         views.index,