diff --git a/AUTHORS b/AUTHORS
index 044391e4a3..4b1cc7d357 100644
--- a/AUTHORS
+++ b/AUTHORS
@@ -196,6 +196,7 @@ answer newbie questions, and generally made Django that much better:
     btoll@bestweb.net
     C8E
     Caio Ariede <caio.ariede@gmail.com>
+    Caitie Baca <caitlin.baca@yahoo.com>
     Calvin Spealman <ironfroggy@gmail.com>
     Cameron Curry
     Cameron Knight (ckknight)
diff --git a/django/test/testcases.py b/django/test/testcases.py
index 5f0c819815..c587f770a6 100644
--- a/django/test/testcases.py
+++ b/django/test/testcases.py
@@ -43,6 +43,7 @@ from django.db.backends.base.base import NO_DB_ALIAS, BaseDatabaseWrapper
 from django.forms.fields import CharField
 from django.http import QueryDict
 from django.http.request import split_domain_port, validate_host
+from django.template.base import PartialTemplate
 from django.test.client import AsyncClient, Client
 from django.test.html import HTMLParseError, parse_html
 from django.test.signals import template_rendered
@@ -138,10 +139,22 @@ class _AssertTemplateUsedContext:
         self.rendered_templates.append(template)
         self.context.append(copy(context))
 
+    @property
+    def rendered_template_names(self):
+        return [
+            (
+                f"{t.origin.template_name}#{t.name}"
+                if isinstance(t, PartialTemplate)
+                else t.name
+            )
+            for t in self.rendered_templates
+            if t.name is not None
+        ]
+
     def test(self):
         self.test_case._assert_template_used(
             self.template_name,
-            [t.name for t in self.rendered_templates if t.name is not None],
+            self.rendered_template_names,
             self.msg_prefix,
             self.count,
         )
@@ -159,11 +172,8 @@ class _AssertTemplateUsedContext:
 
 class _AssertTemplateNotUsedContext(_AssertTemplateUsedContext):
     def test(self):
-        rendered_template_names = [
-            t.name for t in self.rendered_templates if t.name is not None
-        ]
         self.test_case.assertFalse(
-            self.template_name in rendered_template_names,
+            self.template_name in self.rendered_template_names,
             f"{self.msg_prefix}Template '{self.template_name}' was used "
             f"unexpectedly in rendering the response",
         )
diff --git a/tests/test_utils/templates/template_used/partials.html b/tests/test_utils/templates/template_used/partials.html
new file mode 100644
index 0000000000..028c2d8417
--- /dev/null
+++ b/tests/test_utils/templates/template_used/partials.html
@@ -0,0 +1,3 @@
+{% partialdef hello %}
+<p>Hello</p>
+{% endpartialdef %}
diff --git a/tests/test_utils/tests.py b/tests/test_utils/tests.py
index 9c22b61b4f..70cca3d441 100644
--- a/tests/test_utils/tests.py
+++ b/tests/test_utils/tests.py
@@ -646,6 +646,53 @@ class AssertTemplateUsedContextManagerTests(SimpleTestCase):
             self.assertTemplateNotUsed(response, "template.html")
 
 
+@override_settings(ROOT_URLCONF="test_utils.urls")
+class AssertTemplateUsedPartialTests(SimpleTestCase):
+    def test_template_used_pass(self):
+        with self.assertTemplateUsed("template_used/partials.html#hello"):
+            render_to_string("template_used/partials.html#hello")
+
+    def test_template_not_used_pass(self):
+        with self.assertTemplateNotUsed("hello"):
+            render_to_string("template_used/partials.html#hello")
+
+    def test_template_used_fail(self):
+        msg = "Template 'hello' was not a template used to render the response."
+        with (
+            self.assertRaisesMessage(AssertionError, msg),
+            self.assertTemplateUsed("hello"),
+        ):
+            render_to_string("template_used/base.html")
+
+    def test_template_not_used_fail(self):
+        msg = (
+            "Template 'template_used/partials.html#hello' was used "
+            "unexpectedly in rendering the response"
+        )
+        with (
+            self.assertRaisesMessage(AssertionError, msg),
+            self.assertTemplateNotUsed("template_used/partials.html#hello"),
+        ):
+            render_to_string("template_used/partials.html#hello")
+
+    def test_template_not_used_pass_non_partial(self):
+        with self.assertTemplateNotUsed(
+            "template_used/base.html#template_used/base.html"
+        ):
+            render_to_string("template_used/base.html")
+
+    def test_template_used_fail_non_partial(self):
+        msg = (
+            "Template 'template_used/base.html#template_used/base.html' was not a "
+            "template used to render the response."
+        )
+        with (
+            self.assertRaisesMessage(AssertionError, msg),
+            self.assertTemplateUsed("template_used/base.html#template_used/base.html"),
+        ):
+            render_to_string("template_used/base.html")
+
+
 class HTMLEqualTests(SimpleTestCase):
     def test_html_parser(self):
         element = parse_html("<div><p>Hello</p></div>")